Thermolabile UNG (Uracil N-Glycosylase) protein

• Catalog Number: orb533367
• Category: Tools
• Description: Thermolabile UNG is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions. Uracyl N-Glycosylase (UNG, UDG) catalyses the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures. An amount of 1 unit UNG completely digests 104to 106copies of U-containing DNA fragments in 2 min at 50°C.
• Concentration: 1 unit/µl
• Form/Appearance: liquid (Supplied in 20 mM Tris-HCl pH 8.0, 50 mM NaCl, 1 mM EDTA, 1 mM DTT, 100 µg/ml rAlbumin and 50% [v/v] glycerol)
• Storage: store at -20 °C
• Note: For research use only
• Expiration Date: 6 months from date of receipt.