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Catalog Number | orb1252263 |
---|---|
Category | Antibodies |
Description | THBD Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | THBD/1782 |
Tested applications | ELISA, FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG2a, kappa |
Immunogen | Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this Thrombomodulin antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 0.5-1 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTELISA: 2-4 ug/ml; order the BSA-free formulation for coatingThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thrombomodulin antibody to be titered up or down for optimal performance. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | THBD |
UniProt ID | P07204 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 0.5-1 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTELISA: 2-4 ug/ml; order the BSA-free formulation for coatingThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thrombomodulin antibody to be titered up or down for optimal performance. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human cervical carcinoma with Thrombomodulin antibody (clone THBD/1782). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human placenta with Thrombomodulin antibody (clone THBD/1782). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE Analysis of Purified, BSA-Free Thrombomodulin Antibody (clone THBD/1782). Confirmation of Integrity and Purity of the Antibody.
ELISA, FC, ICC, IF, IHC, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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