Th-Pok Antibody / ZBTB7B

• Catalog Number: orb2636849
• Category: Antibodies
• Description: TH-POK (T-helper-inducing POZ/Kr ppel-like factor), also known as zinc finger protein 67 (ZFP67), zinc finger and BTB domain-containing protein 7B or Kr ppel-related zinc finger protein cKrox, is a 539 amino acid protein that contains one BTB (POZ) domain and four C2H2-type zinc fingers. Localized to the nucleus, TH-POK functions primarily as a key regulator of lineage commitment of immature T-cell precursors. Specifically, the presence of TH-POK directs positively selected thymocytes to the CD4 lineage, whereas its absence causes default development to the CD8 lineage. TH-POK also functions as a transcriptional repressor of various other genes, such as COL1A1, COL1A2 and Fibronectin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-ZBTB7B-1F7
• Tested applications: FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant full-length human Zinc finger and BTB domain-containing protein 7B protein was used as the immunogen for the ZBTB7B antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This ZBTB7B antibody is available for research use only.
• UniProt ID: O15156
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the ZBTB7B antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells using ZBTB7B antibody (green, clone PCRP-ZBTB7B-1F7) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with ZBTB7B antibody (blue, clone PCRP-ZBTB7B-1F7), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using ZBTB7B antibody (clone PCRP-ZBTB7B-1F7). These results demonstrate the foremost specificity of the PCRP-ZBTB7B-1F7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free ZBTB7B antibody (PCRP-ZBTB7B-1F7) as confirmation of integrity and purity.