Cart summary

You have no items in your shopping cart.

    TGN46 Rabbit Monoclonal Antibody

    Catalog Number: orb548420

    DispatchUsually dispatched within 5-10 working days
    $ 520.00
    Catalog Numberorb548420
    CategoryAntibodies
    DescriptionTGN46 Rabbit Monoclonal Antibody
    Species/HostRabbit
    ClonalityMonoclonal
    Clone NumberAOHO-20
    Tested applicationsIHC, IP, WB
    ReactivityHuman
    IsotypeRabbit IgG
    ImmunogenA synthesized peptide derived from human TGN46
    ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
    Form/AppearanceLiquid
    ConjugationUnconjugated
    MW51113 MW
    UniProt IDO43493
    StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
    Alternative namesTrans-Golgi network integral membrane protein 2;TG
    Read more...
    NoteFor research use only
    Application notesWB 1:500-1:2000IHC 1:50-1:200IP 1:50
    Expiration Date12 months from date of receipt.
    TGN46 Rabbit Monoclonal Antibody

    Western blot analysis of TGN46 expression in HeLa cell lysate (orb548420). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGOLN2 monoclonal antibody (Catalog # orb548420) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for TGOLN2.

    Submit a review

    Filter by Rating

      • Star
      • Star
      • Star
      • Star
      • Star
      • 5 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 4 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 3 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 2 stars
      • Star
      • Star
      • Star
      • Star
      • Star
      • 1 stars