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Catalog Number | orb1262925 |
---|---|
Category | Antibodies |
Description | TGM2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This Transglutaminase (TGM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-458 amino acids from the Central region of human Transglutaminase (TGM2). |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 77 kDa |
Target | TGM2 |
UniProt ID | P21980 |
NCBI | P21980 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Protein-glutamine gamma-glutamyltransferase 2, Tis Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in HUVEC, K562 cell line lysates (35 ug/lane).This demonstrates the TGM2 antibody detected the TGM2 protein (arrow).
Fluorescent confocal image of U251 cell stained with.U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TGM2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).TGM2 immunoreactivity is localized to Cytoplasm significantly.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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