TGFA Antibody

• Catalog Number: orb1252696
• Category: Antibodies
• Description: TGFA Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MF9
• Tested applications: FC, IF, IHC, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Isotype: IgG1, kappa
• Immunogen: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TGFA
• UniProt ID: P01135
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: TFGAProtransforming growth factor alpha, EGF-like
• Note: For research use only
• Application notes: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human testicular carcinoma with TGF alpha antibody.