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| Catalog Number | orb1040886 |
|---|---|
| Category | Antibodies |
| Description | TGF beta Receptor I Mouse Monoclonal Antibody |
| Target | TGFBR1 |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | Mouse IgG2a, Kappa |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Purification | Affinity purified by Protein A |
| Immunogen | KLH conjugated synthetic peptide derived from human TGF beta Receptor I |
| UniProt ID | P36897 |
| MW | 55 kDa |
| Tested applications | IF, IHC-Fr, IHC-P |
| Dilution range | IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500 |
| Antibody Type | Primary Antibody |
| Clone Number | 5A8 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | TGF-beta receptor type-1; TGFBR1; TGF beta R1; AAT Read more... |
| Research Area | Hypoxia, Molecular Biology, Surface Molecules |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TGF beta Receptor I) Monoclonal Antibody, Unconjugated (orb1040886) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TGF beta Receptor I) Monoclonal Antibody, Unconjugated (orb1040886) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TGF beta Receptor I) Monoclonal Antibody, Unconjugated (orb1040886) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.