TGF beta 1 antibody

• Catalog Number: orb76826
• Category: Antibodies
• Description: Mouse monoclonal antibody to TGF beta 1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TB21
• Tested applications: ELISA, IHC-Fr, IHC-P, WB
• Reactivity: Bovine, Human, Mink, Mouse, Rabbit, Rat, Sheep
• Isotype: IgG1
• Immunogen: TGF-beta from human platelets
• Concentration: 1mg/ml
• Dilution range: Neutralizing: This antibody neutralizes TGF-beta activity in vitro, in an inhibition assay of CCL/64 cell growth and neutralizes the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. IHC: This antibody has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma at a dilution of 1:1000. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Western Blotting: This antibody when used at an antibody concentration of 5--20 ng/mL will allow visualization of 100 ng/lane of TGF-beta.
• Conjugation: Unconjugated
• Target: TGF beta1
• UniProt ID: P01137
• Buffer/Preservatives: Whole IgG in 0.01M PBS, pH 7.0
• Alternative names: Camurati Engelmann disease antibody;CED antibody;D
• Note: For research use only
• Application notes: Sp2/0 Ag.14
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TGF beta-21 using TGF beta antibody