TGF alpha Antibody [MF9]

• Catalog Number: orb1252696
• Category: Antibodies
• Description: TGF alpha Antibody [MF9]
• Target: TGFA
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.
• UniProt ID: P01135
• Tested applications: FC, IF, IHC, WB
• Application notes: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
• Antibody Type: Primary Antibody
• Clone Number: MF9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TFGAProtransforming growth factor alpha, EGF-like
• Research Area: Signal Transduction
• Note: For research use only

IHC testing of FFPE human testicular carcinoma with TGF alpha antibody.