TFRC Mouse Monoclonal Antibody

• Catalog Number: orb2283916
• Category: Antibodies
• Description: TFRC Mouse Monoclonal Antibody
• Target: TFRC
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Recombinant human CD71 protein (89-760/760aa)
• UniProt ID: P02786
• MW: 85 kDa
• Tested applications: FC, ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1：50, Flow-Cyt=2ug/Test
• Antibody Type: Primary Antibody
• Clone Number: 5D12
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ansferrin receptor protein 1; TFRC; CD 71; CD71 an
• Note: For research use only

Blank control: Hela. Primary Antibody (green line): Mouse Anti-TFRC antibody (orb2283916), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (TFRC) monoclonal Antibody, Unconjugated (orb2283916) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human HT-1080 cell lysates, Primary: Anti-TFRC (orb2283916) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 74/85 kDa, Observed band size: 95 kDa.