TFRC Mouse Monoclonal Antibody

SKU: orb2283916

Description

TFRC Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=2ug/Test
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 5D12
• Immunogen: Recombinant human CD71 protein (89-760/760aa)
• Target: TFRC
• Molecular Weight: 85 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ansferrin receptor protein 1; TFRC; CD 71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TR; sTfR; Transferrin receptor(p90 CD71); Transferrin Receptor Protein 1; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; TRFR; TFR1_HUMAN.

Blank control: Hela. Primary Antibody (green line): Mouse Anti-TFRC antibody (orb2283916), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (TFRC) monoclonal Antibody, Unconjugated (orb2283916) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human HT-1080 cell lysates, Primary: Anti-TFRC (orb2283916) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 74/85 kDa, Observed band size: 95 kDa.

Quick Database Links

Gene Symbol

TFRC

UniProt

P02786