TFAP2C antibody

• Catalog Number: orb19002
• Category: Antibodies
• Description: Goat polyclonal antibody to TFAP2C
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:128000, WB: 0.1 μg/ml, IHC-P: 2-3µg/ml
• Conjugation: Unconjugated
• MW: 49.2
• Target: AP-2 gamma
• Entrez: 7022
• Protein Sequence: NKTLEKMEKHRK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TFAP2C antibody, anti ERF1 antibody, anti TFA
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 2-3ug/ml.WB: WB experiments gave bands at approx 75kDa and 22kDa in Human Placenta and Ovary lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.2kDa according to NP_003213.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19002 (2.5ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.