Tetanus Toxin fragment C antibody [Out of stock]

• Catalog Number: orb1136274
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Tetanus Toxin fragment C
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Other
• Isotype: IgG
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a polyhistidine-tagged TTFC fusion protein expressed in E. coli corresponding to full length TTFC. The polyhistidine-tag was placed at the amino terminus of the protein. The recombinant protein was purified from soluble bacterial extracts by metal-affinity and ion-exchange chromatography.
• Concentration: 1ug/ul
• Dilution range: This antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. For western blot experiments, one can expect a band of ≈52 kDa in size corresponding to full-length TTFC. For direct ELISA, coat plates with 1 μg of recombinant TTFC (0.1ml/well). Antigen-specific signal can be detected at a dilution up to 1:10,000,000. For antigen capture ELISA, 0.1 ml of the diluted antisera (1:4000) was able to detect 6 picograms of TTFC using monoclonal mouse anti-TTFC as the antigen-detection antibody (0.5 μg/ml, Roche Biosciences). Sera diluted up to 100,000-fold can easily detect 600 picograms of recombinant TTFC by Western blot processed with ECL Plus Western Blotting and Detection System (Amersham Biosciences, 1 sec film exposure).
• Form/Appearance: Liquid
• Purity: Purified
• Conjugation: Unconjugated
• Storage: This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
• Buffer/Preservatives: PBS, pH 7.4 with 0.05% sodium azide.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.