Terminal Deoxynucleotidyl Transferase (TdT)

SKU: orb1173258

Description

Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that catalyzes nucleotide addition to 3' DNA termini. Primarily targeting 3'-overhangs, this specialized enzyme also modifies blunt or recessed ends, serving as a vital tool for non-templated DNA synthesis.

Research Area

Signal Transduction

Key Properties

• Source/Expression System: E. coli
• Protein Sequence: Amino acid sequence derived from Bovine TdT (X04122) (Mer1-Ala520) was expressed.
• Molecular Weight: 58.3 kDa
• Purity: > 90% as determined by SDS-PAGE
• Protein Length: The recombinant bovine TdT consists of 509 amino acids and migrates with an apparent molecular mass of 58.3 kDa as estimated in SDS-PAGE under reducing conditions.

Storage & Handling

• Storage: Stored at -20°C.
• Form/Appearance: The recombinant bovine TdT reconstitution solution, the compound solution is a sterile aqueous solution, 50 mM K2HPO4, 100 mM NaCl, 1 mM DTT, 0.1% Tween20, 1% rAlbumin, 50% Glycerol, pH 6.5.
• Buffer/Preservatives: Liquid in sterile 50 mM K2HPO4, 100 mM NaCl, 1 mM DTT, 0.1% Tween20, 1% rAlbumin, 50% Glycerol, pH 6.5.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
• Disclaimer: For research use only

Alternative Names

DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase