Tenascin C Antibody

• Catalog Number: orb749580
• Category: Antibodies
• Description: Tenascin C is a multifunctional, disulfide-linked hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM319
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human breast carcinoma cells were used as the immunogen for the anti-Tenascin C antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This anti-Tenascin C antibody is available for research use only.
• UniProt ID: P24821
• Storage: Store the anti-Tenascin C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the anti-Tenascin C antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with anti-Tenascin C antibody (clone SPM319) at 4 ug/ml.

SDS-PAGE analysis of purified, BSA-free anti-Tenascin C antibody (clone SPM319) as confirmation of integrity and purity.