Tenascin antibody

• Catalog Number: orb44503
• Category: Antibodies
• Description: Mouse Monoclonal to Tenascin.
• Clonality: Monoclonal
• Clone Number: T2H5
• Tested applications: IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Protein preparation from a homogenate of a human mammary tumour specimen.
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Immunohistochemical detection of tenascin is valuable for studies of tissue differentiation and tumour growth. The antibody T2H5 is excellent for staining of paraffin-embedded tissue sections.Western blotting: Recommended dilution: 1-2 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Tenascin C
• Entrez: 3371
• UniProt ID: P24821
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: Tenascin
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Immunohistochemical detection of tenascin is valuable for studies of tissue differentiation and tumour growth. The antibody T2H5 is excellent for staining of paraffin-embedded tissue sections. Heat-mediated antigen retrieval in citrate buffer.Western blotting: Recommended dilution: 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of U-87 MG cells stained using anti-tenascin C (T2H5) purified antibody (concentration in sample 12 µg/ml, GAM FITC, red-filled) from U-87 MG cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (surface staining).

Immunocytochemistry staining of tenascin C in U-87 MG cells using purified mouse monoclonal antibody T2H7 (concentration in sample 12 µg/ml, GAM FITC, right picture) vs. Hoechst 34580 nuclear staining (left picture).

Immunohistochemistry staining of tonsil (paraffin-embedded sections) with anti-human tenascin C (T2H5).

Western blotting analysis of human tenascin C using mouse monoclonal antibody T2H5 on lysates of U87-MG cell line and Jurkat cell line (tenascin non-expressing cell line; negative control) under non-reducing and reducing conditions. Cells were lysed by 50 mM TRIS-Cl pH 6.6, 4M urea, 4% SDS, samples were mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer, then resolved using 7.5% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-tenascin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Tenascin C was detected slightly above 250 kDa.