TDRD3 Antibody

• Catalog Number: orb1671525
• Category: Antibodies
• Description: TDRD3 Antibody
• Clonality: Recombinant
• Clone Number: RAB-C419
• Tested applications: ChIP, ELISA, IF
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing TDRD3 protein under non-denaturing conditions.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: TDRD3
• UniProt ID: Q9H7E2
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Tudor domain-containing protein 3, anti-TDRD3-RAB-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-TDRD3 antibody R Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Western Blot using anti-TDRD3 antibody R Human brain cerebellum(A) (1 ug/ml)- human skeletal muscle(B) (0.3 ug/ml)- heart(C) (0.3 ug/ml)- human placenta(D) (0.1 ug/ml)- human testes(E) (0.3 ug/ml) and human kidney(F) (0.1 ug/ml) tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescence staining of HeLa cells with anti-TDRD3. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution)- showing nucleoli- nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671525- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.