TDP43 Antibody / TARDBP / TAR DNA binding protein

• Catalog Number: orb1824482
• Category: Antibodies
• Description: TARDBP (TAR DNA binding protein), also known as TDP-43, is a nuclear protein that contains two RRM (RNA recognition motif) domains. Ubiquitously expressed with highest levels found in placenta, lung, pancreas, spleen and genital tract, TARDBP functions as a DNA-binding protein and specifically binds to the TAR DNA sequence motifs of HIV. Via this association with TAR motifs, TARDBP acts as a transcriptional repressor and inhibits HIV-1 transcription. TARDBP can also function as a negative regulator of splicing activity and is known to be involved in the splicing of CFTR (cystic fibrosis transmembrane receptor). In addition, TARDBP is a major component of ubiquitin-positive inclusion bodies that are prominent in many neurodegenerative diseases. This suggests that TARDBP may play a role in the development of neurodegenerative disorders. Due to alternative splicing events, various isoforms exist for TARDBP.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant fragment from the human protein (within amino acids 200-414) was used as the immunogen for the TARDBP antibody.
• UniProt ID: Q13148
• Tested applications: ELISA, IHC-P, WB
• Dilution range: ELISA (Order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: TARDP/349
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Aliquot the TARDBP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This TARDBP antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human prostate tissue with TARDBP antibody (clone TARDP/349). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free TARDBP antibody (clone TARDP/349) as confirmation of integrity and purity.