TCR gamma/delta Antibody

• Catalog Number: orb1088491
• Category: Antibodies
• Description: The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
• Clonality: Monoclonal
• Clone Number: 11F2
• Tested applications: ELISA, FC, IHC-Fr, IP
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified CD3 antigen complex
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: TCR gamma/delta
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-TCRG/D antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of TCR gamma/delta positive lymphocytes (red-filled) from human TCR gamma/delta negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human TCR gamma/delta (11F2) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).