TCR alpha antibody (PE )

• Catalog Number: orb154506
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to PE which recognizes TCR alpha that recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex.
• Clonality: Monoclonal
• Clone Number: R73
• Tested applications: FC
• Reactivity: Primate, Rat
• Isotype: Mouse IgG1 kappa
• Immunogen: Rat T blasts and erythrocytes
• Concentration: 0.5 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: TCR alpha/beta
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-TCRA/B antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of rat thymocytes stained using anti-rat TCR alpha/beta (R73) PE antibody (concentration in sample 0.11 µg/ml, red) from unstained rat thymocytes (black) in flow cytometry analysis (surface staining).