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Catalog Number | orb371727 |
---|---|
Category | Antibodies |
Description | TCP1 theta/CCT8 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, IHC-Fr, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human CCT8 recombinant protein (Position: K421-D548). Human CCT8 shares 98.4% amino acid (aa) sequence identity with mouse CCT8. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 59621 MW |
UniProt ID | P50990 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | T-complex protein 1 subunit theta;TCP-1-theta;CCT- Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of CCT8 using anti-CCT8 antibody.Lane 1:rat brain tissue; 2:mouse brain tissue;3:U20S cell.
IHC analysis of CCT8 using anti-CCT8 antibody. CCT8 was detected in a paraffin-embedded section of mouse kidney tissue.
IHC analysis of CCT8 using anti-CCT8 antibody. CCT8 was detected in a paraffin-embedded section of rat testis tissue.
IHC analysis of CCT8 using anti-CCT8 antibody. CCT8 was detected in a paraffin-embedded section of human mammary cancer tissue.
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