TCP1 delta/CCT4 Antibody

• Catalog Number: orb371661
• Category: Antibodies
• Description: TCP1 delta/CCT4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human CCT4 recombinant protein (Position: R452-R539). Human CCT4 shares 97.7% amino acid (aa) sequence identity with both mouse and rat CCT4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 57924 MW
• UniProt ID: P50991
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: T-complex protein 1 subunit delta;TCP-1-delta;CCT-
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of PC-3 cells using anti-CCT4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control

Flow Cytometry analysis of U251 cells using anti-CCT4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of K562 cells using anti-CCT4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CCT4 using anti-CCT4 antibody.Lane 1:human HeLa cell;2:human U20S cell;3:human SW620 cell;4:human MCF-7 cell;5:rat brain tissue;6:rat liver tissue;7:mouse brain tissue;8:mouse liver tissue.

IF analysis of CCT4 using anti-CCT4 antibody.CCT4 was detected in immunocytochemical section of U20S cell.

IHC analysis of CCT4 using anti-CCT4 antibody.CCT4 was detected in paraffin-embedded section of mouse kidney tissues.

IHC analysis of CCT4 using anti-CCT4 antibody.CCT4 was detected in paraffin-embedded section of rat kidney tissues.

IHC analysis of CCT4 using anti-CCT4 antibody.CCT4 was detected in paraffin-embedded section of human mammary cancer tissues.

IHC analysis of TCP1 delta using anti-TCP1 delta antibody.TCP1 delta was detected in frozen section of rat brain tissue.

IHC analysis of TCP1 delta using anti-TCP1 delta antibody.TCP1 delta was detected in immunocytochemical section of SHG-44 cell.

IHC analysis of TCP1 delta using anti-TCP1 delta antibody.TCP1 delta was detected in immunocytochemical section of Neuro-2a cell.

IHC analysis of TCP1 delta using anti-TCP1 delta antibody.TCP1 delta was detected in frozen section of mouse brain tissue.

IHC analysis of TCP1 delta using anti-TCP1 delta antibody.TCP1 delta was detected in immunocytochemical section of LOVO cell.

IHC analysis of TCP1 delta using anti-TCP1 delta antibody.TCP1 delta was detected in immunocytochemical section of NRK cell.