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Catalog Number | orb421123 |
---|---|
Category | Antibodies |
Description | TCP1 alpha Antibody (monoclonal, 2E7) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2E7 |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human TCP1 alpha (515-551aa KFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHS), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 60 kDa |
UniProt ID | P17987 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | T-complex protein 1 subunit alpha; TCP-1-alpha; CC Read more... |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of HepG2 cells using anti-TCP1 alpha antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of TCP1 alpha using anti-TCP1 alpha antibody.Lane 1:human HeLa cell;2:human MCF-7 cell;3:human COLO-320 cell;4:human HepG2 cell;5:human A431 cell;6:human HT1080 cell.
IF analysis of TCP1 alpha using anti-TCP1 alpha antibody. TCP1 alpha was detected in immunocytochemical section of MCF7 cells.
IHC analysis of TCP1 alpha using anti-TCP1 alpha antibody.TCP1 alpha was detected in paraffin-embedded section of human lung cancer tissue.
IHC analysis of TCP1 alpha using anti-TCP1 alpha antibody.TCP1 alpha was detected in paraffin-embedded section of human placenta tissue.
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