TCID

SKU: orb1300546

Description

TCID is a potent and selective inhibitor of ubiquitin C-terminal hydrolase L3 (UCH-L3) with an IC50 of 0.6 µM, demonstrating 125-fold selectivity over UCH-L1. This small molecule probe is used in biochemical and cellular studies to investigate deubiquitinase function, protein homeostasis, and related disease pathways in vitro.

Research Area

Cancer Biology, Protein Biochemistry

Key Properties

• CAS Number: 30675-13-9
• MW: 283.93
• Purity: >98%
• Formula: C₉H₂Cl₄O₂
• SMILES: ClC1=C2C(=O)CC(=O)C2=C(Cl)C(Cl)=C1Cl
• Target: DUB (Deubiquitinating enzyme)
• Solubility: Soluble in DMSO (up to 25 mg/ml),

Bioactivity

• Target IC50:
  UCHL3:0.6 μM
• In Vitro:
  TCID is selective for UCH-L3 over UCH-L1 by over 100-fold. NU6027 (10 μM) does not promote YFP-GLT-1 accumulation in intracellular vesicles in transfected MDCK cells, while LDN-57444 (10 μM) promotes YFP-GLT-1 accumulation. NU6027 (10 μM) does not produce long-term lysosomal degradation of GLT-1, while LDN-57444 (10 μM) has such effect. TCID (10 μM) diminishes GlyT2 ubiquitination in brainstem and spinal cord primary neurons, which is more pronounced when UCHL1 is inhibited and when cells are exposed to these inhibitors for longer periods.
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Storage & Handling

• Storage: RT
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C-terminal, DUBs, Deubiquitinase, glycine, GlyT2, hydrolase, Inhibitor, inhibit, Neuronal, TCID, UCH-L1, UCH-L3 Inhibitor, UCH-L3, ubiquitination, ubiquitin, transporter

Compound Identifiers

PubChem CID

2729042