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    TCFL5 antibody

    Catalog Number: orb19348

    DispatchUsually dispatched within 5-10 working days
    $ 502.00
    Catalog Numberorb19348
    CategoryAntibodies
    DescriptionGoat polyclonal antibody to TCFL5
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, WB
    ReactivityCanine, Human, Mouse, Rat
    Dilution rangeELISA: 1:64000, WB: 0.3-1 μg/ml
    ConjugationUnconjugated
    MW52.7
    TargetCHA / TCFL5
    Entrez10732
    Protein SequenceKYIQERHGDSLKKE
    RRIDAB_10748767
    StorageAliquot and store at -20°C. Minimize freezing and thawing.
    Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
    Alternative namesanti TCFL5 antibody, anti CHA antibody, anti trans
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    NoteFor research use only
    Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 55kDa band observed in Human Testis lysates (calculated MW of 52.7kDa according to NP_006593.2). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was greatly reduced by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
    Expiration Date12 months from date of receipt.
    TCFL5 antibody

    orb19348 (0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

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