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Catalog Number | orb19418 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to TCF3 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Dilution range | ELISA: 1:128000, WB: 0.01-0.03 μg/ml, IHC-P: 3-5µg/ml |
Conjugation | Unconjugated |
MW | 67.6 |
Target | TCF3 / ITF1 |
Entrez | 6929 |
Protein Sequence | KAPRARTSPDEDED |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti TCF3 antibody, anti ITF1 antibody, anti trans Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5ug/ml.WB: Approx 75kDa band observed in lysates of cell line Daudi (calculated MW of 67.6kDa according to NP_003191.1). Recommended concentration: 0.01-0.03 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19418 (0.01ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb19418 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Gallus | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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