TBP1 Antibody

• Catalog Number: orb1524643
• Category: Antibodies
• Description: TBP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 389 and 439
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: TBP1
• UniProt ID: P17980
• NCBI: NP_002795.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: 26S proteasome regulatory subunit 6A; TBP-1; TBP1;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human TBP1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, MCF-7, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TBP1 antibody (orb1524643) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of mouse TBP1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TBP1 antibody (orb1524643) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human TBP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBP1 antibody (orb1524643) used for IP at 6 µg per reaction. TBP1 was also immunoprecipitated by a previous lot of this antibody (orb1524643) and a second antibody against a different epitope of TBP1.

Detection of human TBP1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TBP1 (orb1524643) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB