TBP Mouse Monoclonal Antibody

SKU: orb500348

Description

TBP Mouse Monoclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 6G8
• Immunogen: Recombinant human TBP protein
• Target: TBP
• Molecular Weight: 38 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GTF2D; GTF2D1; HDL4; SCA17; TBP1; TFIID; TBP_CHICK; TBP; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit; TBP_HUMAN; TF2D; TBP_MOUSE;

Paraformaldehyde-fixed, paraffin embedded (rat testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TATA binding protein) Monoclonal Antibody, Unconjugated (orb500348) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: U87MG (Human) Cell Lysate at 30 ug, Primary: Anti-TBP (orb500348) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 38 kD, Observed band size: 40 kD.

Quick Database Links

Gene Symbol

TBP

UniProt

P20226