TBC1D10C Antibody

• Catalog Number: orb1246743
• Category: Antibodies
• Description: TBC1D10C Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, WB
• Predicted Reactivity: Canine, Porcine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-PRRKPQTRGKTFH
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48-50kDa band observed in lysates of cell line Jurkat and in Mouse Thymus lysates and approx 50kDa in Peripheral Blood Mononuclear (PBM) and in Human Spleen lysates (calculated MW of 49.7Da according to Human NP_940919.1 and 49.9kDa according to Mouse NP_848765.2). Recommended concentration:0.3-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 cell lysate.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TBC1D10C
• UniProt ID: Q8IV04
• NCBI: NP_940919.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TBC1D10C, carabin, TBC1 domain family, member 10C,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48-50kDa band observed in lysates of cell line Jurkat and in Mouse Thymus lysates and approx 50kDa in Peripheral Blood Mononuclear (PBM) and in Human Spleen lysates (calculated MW of 49.7Da according to Human NP_940919.1 and 49.9kDa according to Mouse NP_848765.2). Recommended concentration:0.3-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 cell lysate.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246743 (0.5 ug/ml) staining of Jurkat (A), (0.3 ug/ml) PBM (B) and negative control A431 (C) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246743 (2 ug/ml) staining of Human Spleen (A) and Mouse Thymus (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246743 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).