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Catalog Number | orb1294394 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Tau |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
Dilution range | Western Blot 1:3000 – 1:5000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:200 – 1:300 Immunohistochemistry (Frozen) 1:200 – 1:400 |
Conjugation | Unconjugated |
Target | Tau |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescent analysis of primary cortical neurons.Tau antibody : 1:300;Anti-rabbit 488 : 1:500.
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling Tau with orb1294394 at 1:200.
Immunohistochemical of frozen sections.Sample : mouse cerebellum;Green : Tau antibody : 1:300;Anti-rabbit 488 : 1:500;DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence:cells were stained with anti-Tau antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Western blot analysis using Anti-Tau antibody, lysates/proteins at 45 μg per lane.
Immunofluorescent analysis of primary cortical neurons, using Tau antibody at 1:300, Anti-rabbit 488 at 1:500.
Immunohistochemical analysis of paraffin embedded Human astroglioma tissue labeling Tau with orb1294394 at 1:300.
ELISA, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IHC-Fr, IHC-P, WB | |
Human, Monkey, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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