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Item 1 of 4
Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils
Catalog Number: orb1822357
| Catalog Number | orb1822357 |
|---|---|
| Category | Proteins |
| Description | Human Recombinant Tau-441 (2N4R) P301S Mutant Protein Pre-formed Fibrils |
| Tested applications | In vitro, In vivo, SDS-PAGE, WB |
| Reactivity | Human |
| Tag | No tag |
| Concentration | 2 mg/ml |
| Purity | > 95% |
| MW | ~45.8 kDa |
| Target | Tau-441 (2N4R) P301S Mutant |
| UniProt ID | P10636 |
| Protein Sequence | MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEDVTAPLV DEGAPGKQAA AQPHTEIPEG TTAEEAGIGD TPSLEDEAAG HVTQARMVSK SKDGTGSDDK KAKGADGKTK IATPRGAAPP GQKGQANATR IPAKTPPAPK TPPSSGEPPK SGDRSGYSSP GSPGTPGSRS RTPSLPTPPT REPKKVAVVR TPPKSPSSAK SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVSGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE NAKAKTDHGA EIVYKSPVVS GDTSPRHLSN VSSTGSIDMV DSPQLATLAD EVSASLAKQG L |
| Protein Length | Full Length |
| Source | Recombinant |
| Expression System | E. coli |
| Biological Activity | Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers. |
| Storage | -80°C |
| Buffer/Preservatives | 10 mM HEPES, 100 mM NaCl pH 7.4 |
| Alternative names | Tau PFFs, Tau PFF, Tau protein Pre-formed Fibrils, Read more... |
| Note | For research use only |
| Application notes | Certified > 95% pure using SDS-PAGE analysis. Low endotoxin < 5 EU/mL @ 2mg/mL. |
| Expiration Date | 6 months from date of receipt. |
TEM of recombinant Tau441 (2N4R), P301S mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV = 80kV. Fibrils were sonicated and stained with uranyl acetate.
TEM of recombinant Tau441 (2N4R), P301S mutant Pre-formed Fibrils (PFFs). Fibrils were sonicated and stained with uranyl acetate.
Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) when tau Pre-formed Fibrils are combined with tau monomers. The Pre-formed Fibrils seed the formation of new fibrils from a pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.
SDS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Pre-formed Fibrils. Lane 1: MW Ladder. Lane 2: Tau Protein Pre-formed Fibrils
