Tat-SF1 Antibody

• Catalog Number: orb1527372
• Category: Antibodies
• Description: Tat-SF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 375 and 425
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Tat-SF1
• UniProt ID: O43719
• NCBI: NP_055315.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: dJ196E23.2; TATSF1; TAT-SF1; HIV TAT specific fact
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Tat-SF1 by western blot. Samples: Whole cell lysate (10 µg) from Jurkat, HeLa, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Tat-SF1 antibody (orb1527372) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human Tat-SF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Tat-SF1 antibody (orb1527372) used for IP at 6 µg per reaction. Tat-SF1 was also immunoprecipitated by a previous lot of this antibody (orb1527372) and a second antibody against a different epitope of Tat-SF1.