Taq-Polymerase RecAb (TP7)

• Catalog Number: orb2568780
• Category: Antibodies
• Description: Taq-Polymerase RecAb (TP7) is a recombinant mouse antibody (mIgG2a,κ) that binds to Taq polymerase and inhibits the polymerase activity at room temperature. The antibody is inactivated and the enzyme released upon temperature increase (“hot start antibody”) during normal PCR cycling conditions, allowing reactions to be set up at room temperature. The target Taq polymerase (Uniprot: P19821) is produced by the thermophilic eubacterium Thermus aquaticus and is a popular thermostable DNA polymerase used for PCR [1][2][3]. The original TP7 antibody was prepared by Scalice et al. (1994) [4] through mouse immunization with recombinant Taq expressed in E. coli. The recombinant antibody is produced under serum‐free (animal component-free) conditions in CHO cells system and purified through one-step purification with Protein-A affinity chromatography. We recommended to use TP7 antibody at a ≥ 1:1 molar ratio over Taq polymerase.
• Target: Taq-Polymerase
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a κ
• Conjugation: Unconjugated
• Reactivity: Bacteria
• Form/Appearance: Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
• Concentration: ≥ 0.5 mg/ mL
• Buffer/Preservatives: Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
• Purity: ≥ 90% (CGE, reducing conditions). ≤ 10 % aggregates (analytical SEC)
• Immunogen: recombinant TaqPol expressed in E. coli
• UniProt ID: P19821
• Tested applications: PCR
• Antibody Type: Recombinant Antibody
• Clone Number: TP7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DNA polymerase I, thermostable, polA, pol1
• Note: For research use only