TAK1/MAP3K7 Antibody

• Catalog Number: orb371740
• Category: Antibodies
• Description: TAK1/MAP3K7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, WB
• Predicted Reactivity: Bovine, Gallus, Monkey, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TAK1 (566-603aa RLVQEHKKLLDENKSLSTYYQQCKKQLEVIRSQQQKRQ), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 67196 MW
• UniProt ID: O43318
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Mitogen-activated protein kinase kinase kinase 7;2
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U87 cells using anti-TAK1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of TAK1 using anti-TAK1 antibody.Lane 1:human MCF-7 cell; 2:human A431 cell; 3:human A374 cell; 4:rat heart tissue; 5:mouse heart tissue; 6:mouse NIH/3T3 cell.

IF analysis of TAK1 using anti-TAK1 antibody. TAK1 was detected in an immunocytochemical section of SiHa cells.