TAGLN2 Rabbit Polyclonal Antibody

• Catalog Number: orb704391
• Category: Antibodies
• Description: TAGLN2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant human TAGLN2 (1-199/199aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ICC/IF=1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 22 kDa
• Target: TAGLN2
• UniProt ID: P37802
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CDABP0035; epididymis tissue protein Li 7e; HA1756
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (KO Validated) TAGLN2 polyclonal Antibody, Unconjugated (orb704391) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: 293T (Human) Cell Lysate at 25 ug, Lane 2: TAGLN2 knockout (KO) 293T (Human) Cell Lysate at 25 ug, Primary: Anti-TAGLN2 (orb704391) at 1/1000 dilution, Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution, Predicted band size: 18 kD, Observed band size: 20 kD.

Sample: Lane 1: Lung (Mouse) Lysate at 40 ug, Lane 2: Placenta (Mouse) Lysate at 40 ug, Lane 3: Uterus (Mouse) Lysate at 40 ug, Lane 4: U251 (Human) Cell Lysate at 30 ug, Lane 5: A431 (Human) Cell Lysate at 30 ug, Lane 6: U87MG (Human) Cell Lysate at 30 ug, Lane 7: U2os (Human) Cell Lysate at 30 ug, Lane 8: Huvec (Human) Cell Lysate at 30 ug, Lane 9: A673 (Human) Cell Lysate at 30 ug, Primary: Anti-TAGLN2 (orb704391) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kD, Observed band size: 22 kD.