TAGLN Antibody

• Catalog Number: orb1672051
• Category: Antibodies
• Description: TAGLN Antibody
• Clonality: Recombinant
• Clone Number: SAIC-33B-129
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Peptide "AAEDYGVIK", derived from the actin cross-linking protein Transgelin, conjugated to KLH.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: TAGLN
• UniProt ID: Q01995
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Transgelin,SM22,WS3-10
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the antibody SAIC-33B-129. Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SAIC-33B-129 (orb1672051 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-TAGLN (SAIC-33B-129). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-33B-129 (orb1672051) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672051- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-TAGLN antibody SAIC-33B-129. HeLa(A)- Caco-2(B)- U2OS(C) cell lysate and human placenta(D)- muscle(E) tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-33B-129 (orb1672051) at 0.0003 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.