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Item 1 of 2
TAF9 Antibody (N-term)
Catalog Number: orb1935666
| Catalog Number | orb1935666 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB34582 |
| Tested applications | IF, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 28974 Da |
| Target | This TAF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TAF9. |
| UniProt ID | Q16594 |
| NCBI | NP_003178.1, NP_001015892.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Transcription initiation factor TFIID subunit 9, R Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
TAF9 Antibody (N-term) western blot analysis in U251 cell line lysates (35 ug/lane). This demonstrates the TAF9 antibody detected the TAF9 protein (arrow).
Fluorescent image of U251 cell stained with TAF9 Antibody (N-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TAF9 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). TAF9 immunoreactivity is localized to Nucleus significantly.
