TAF1 Antibody

• Catalog Number: orb1524710
• Category: Antibodies
• Description: TAF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 1750 and 1800
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: TAF1
• UniProt ID: P21675
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: Transcription initiation factor TFIID 250 kDa subu
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human TAF1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, HeLa, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAF1 antibody orb1524710 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human TAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAF1 antibody orb1524710 used for IP at 6 µg per reaction.

Detection of human TAF1 by immunohistochemistry.Sample: FFPE section of lung carcinoma.Antibody: Rabbit anti-TAF1 antibody (orb1524710) used at 1: 5000 (0.2 µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human TAF1 by immunohistochemistry.Sample: FFPE section of bladder carcinoma. Antibody: Rabbit anti-TAF1 antibody (orb1524710) used at 1: 1000 (1 µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.