T-bet/Tbx21 Antibody

• Catalog Number: orb137878
• Category: Antibodies
• Description: T-bet/Tbx21 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human T-bet (522-535aa DKEAEGQFYNYFPN), different from the related rat and mouse sequences by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 58328 MW
• UniProt ID: Q9UL17
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: T-box transcription factor TBX21;T-box protein 21;
• Note: For research use only
• Application notes: WB: The detection limit for TBX21 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: Recombinant Human TXB21 Protein 10ng, Lane 2: Recombinant Human TXB21 Protein 5ng, Lane3: Recombinant Human TXB21 Protein 2.5ng using TBX21 antibody