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T-bet Antibody / Tbox-bet / TBX21
Catalog Number: orb1825045
| Catalog Number | orb1825045 |
|---|---|
| Category | Antibodies |
| Description | T helper (Th) lymphocytes differentiate into two unique subsets, Th1 and Th2, which differ both in function and in the cytokines they secrete. Th1 and Th2 cytokines promote the growth and differentiation of their subset, and inhibit the growth and differentiation of the opposing subset. T-bet (T-box expressed in T cells) is a Th1-specific T-box transcription factor that controls the expression of the Th1 cytokine, IFN-g. T-bet also converts effector Th2 cells into the opposing Th1 subset. T-bet is selectively expressed in Th1 cells. The level of T-bet expression is increased by signals mediated by the T cell receptor (TCR). IL-12 also induces an increase in the level of T-bet. T-bet was originally isolated from nuclear extracts of resting and PMA/ionomycin-activated AE7 cells. It is expressed in low levels in AE7 cells, and in increased levels in stimulated AE7. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | TBX21/6724 |
| Tested applications | IHC-P |
| Reactivity | Human |
| Isotype | Mouse IgG1, kappa |
| Immunogen | A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the TBX21 antibody. |
| Antibody Type | Primary Antibody |
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
| Conjugation | Unconjugated |
| Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Hazard Information | This TBX21 antibody is available for research use only. |
| UniProt ID | Q9UL17 |
| Storage | Aliquot the TBX21 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human colon carcinoma tissue with TBX21 antibody (clone TBX21/6724). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
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