Syndecan 1 antibody (APC)

• Catalog Number: orb623578
• Category: Antibodies
• Description: Mouse monoclonal antibody against Syndecan 1 conjugated to APC
• Clonality: Monoclonal
• Clone Number: MI15
• Tested applications: FC
• Reactivity: Human, Primate, Rat
• Isotype: Mouse IgG1 kappa
• Immunogen: A mixture of U266 and XG-1 human myeloma cell lines
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC
• Target: CD138
• Entrez: 6382
• UniProt ID: P18827
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-syndecan 1 antibody, anti-SYND1 antibody, ant
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Staining pattern of Anti-human CD38 FITC antibody (clone HIT2) in dot-plot fluorescence visualization (B cell gate) Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Mouse monoclonal anti-human CD38 FITC antibody (clone HIT2) in concentration 0.5 µg/ml in stained stained blood sample (2 x 10^6 cells) and Mouse monoclonal anti-human CD27 Pacific Blue™ antibody (clone LT27) was used in concentration 2 µg/ml, respectively.

Staining pattern of Anti-human CD38 FITC antibody (clone HIT2) in dot-plot fluorescence visualization (B cell gate) Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Mouse monoclonal anti-human CD38 FITC antibody (clone HIT2) in concentration 0.5 µg/ml in stained stained blood sample (2 x 10^6 cells) and Mouse monoclonal anti-human CD138 APC antibody (clone MI15) in concentration 2 µg/ml, respectively.

Reactivity of Anti-human CD38 FITC antibody (clone HIT2) on human peripheral leukocytes Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Suspension of blood leukocytes (2 x 10^6 cells) was added to the mixture of CD38 FITC antibody (clone HIT2, 0.5 µg/ml in stained blood sample), backbone antibody conjugates and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Stained sample was fixed with 2 ml of 10× diluted EXCELLYSE Easy solution for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Anti-human CD38 FITC antibody (clone HIT2) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Mouse monoclonal anti-human CD38 FITC antibody (clone HIT2) was used in concentration 0.5 µg/ml in stained blood sample (2 x 10^6 cells).