SUZ12 Antibody

• Catalog Number: orb1274421
• Category: Antibodies
• Description: SUZ12 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 83kDa
• Target: SUZ12
• UniProt ID: Q15022
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: SUZ12 Antibody: CHET9, JJAZ1, CHET9, KIAA0160, Pol
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SUZ12 Antibody (orb1274421) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts from normal (control) and SUZ12 knockout (KO) HeLa cells, using SUZ12 antibody (orb1274421) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat lung using SUZ12 antibody (orb1274421) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using SUZ12 antibody (orb1274421) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using SUZ12 antibody (orb1274421) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using SUZ12 antibody (orb1274421) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using SUZ12 antibody (orb1274421) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using SUZ12 antibody (orb1274421) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using SUZ12 Polyclonal Antibody (orb1274421) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA1 Antibody (orb1256354) at dilution of 1:100 (40x lens).