Survivin Antibody / BIRC5

• Catalog Number: orb1824899
• Category: Antibodies
• Description: The baculovirus protein p35 inhibits virally induced apoptosis of invertebrate and mammalian cells and may function to impair the clearing of virally infected cells by the host s immune system. This is accomplished at least in part by its ability to block both TNF- and FAS-mediated apoptosis through the inhibition of the ICE family of serine proteases. Two mammalian homologs of baculovirus p35, referred to as inhibitor of apoptosis protein (IAP) 1 and 2, share an amino terminal baculovirus IAP repeat (BIR) motif and a carboxy terminal RING finger. Although the c-IAPs do not directly associate with the TNF receptor (TNF-R), they efficiently block TNF-mediated apoptosis through their interaction with the downstream TNF-R effectors, TRAF1 and TRAF2. Additional IAP family members include ILP (for IAP-like protein) and survivin. ILP inhibits activated caspase-3, leading to the resistance of FAS-mediated apoptosis. Survivin (also designated TIAP) is expressed during the G2/M phase of the cell cycle and associates with microtubules of the mitotic spindle. Increased caspase-3 activity is detected when a disruption of survivin-microtubule interactions occurs.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BIRC5/8936R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 1-100) from the human protein was used as the immunogen for the recombinant Survivin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This recombinant Survivin antibody is available for research use only.
• UniProt ID: O15392
• Storage: Aliquot the recombinant Survivin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 293T cell lysate with recombinant Survivin antibody (clone BIRC5/8936R). Predicted molecular weight ~16 kDa.

IHC staining of FFPE human ovarian cancer tissue with recombinant Survivin antibody (clone BIRC5/8936R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human testis tissue with recombinant Survivin antibody (clone BIRC5/8936R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Survivin antibody (clone BIRC5/8936R) as confirmation of integrity and purity.