Superoxide Dismutase Antibody Peroxidase Conjugated

• Catalog Number: orb344919
• Category: Antibodies
• Description: Superoxide Dismutase antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: HRP
• Reactivity: Bovine
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: Superoxide Dismutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Superoxide Dismutase [Bovine erythrocytes]. Cross reactivity against Superoxide Dismutase from other tissues and species may occur but have not been specifically determined.
• Immunogen: Superoxide Dismutase [Bovine erythrocytes]
• UniProt ID: P00442
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:5,000 - 1:20,000, WB: 1:500- 1:2,000
• Application notes: Anti-Superoxide Dismutase Peroxidase Conjugated Antibody has been tested by Western blot and is suitable to be assayed against 1.0 ug of Superoxide Dismutase [Bovine erythrocytes] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.
• Antibody Type: Primary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: rabbit anti-Superoxide Dismutase 1 Antibody HRP co
• Note: For research use only
• NCBI: DAA33706.1

Western Blot of HRP Conjugated Superoxide Dismutase Antibody. Lane 1: Superoxide Dismutase Load: 50 ng per lane. Primary antibody: HRP Conjugated Superoxide Dismutase Antibody at 1:1000 overnight at 4°C. Secondary antibody: none Detected directly using FEMTOMAX Substrate. Block: orb348637 for 30 min at RT. Predicted/Observed size: 15.6 kDa, 15.6 kDa.