You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb344919 |
---|---|
Category | Antibodies |
Description | Superoxide Dismutase antibody (Peroxidase) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bovine |
Isotype | IgG |
Immunogen | Superoxide Dismutase [Bovine erythrocytes] |
Antibody Type | Primary Antibody |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:5,000 - 1:20,000, WB: 1:500- 1:2,000 |
Form/Appearance | Lyophilized |
Purity | Superoxide Dismutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Superoxide Dismutase [Bovine erythrocytes]. Cross reactivity against Superoxide Dismutase from other tissues and species may occur but have not been specifically determined. |
Conjugation | HRP |
UniProt ID | P00442 |
NCBI | DAA33706.1 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | rabbit anti-Superoxide Dismutase 1 Antibody HRP co Read more... |
Note | For research use only |
Application notes | Anti-Superoxide Dismutase Peroxidase Conjugated Antibody has been tested by Western blot and is suitable to be assayed against 1.0 ug of Superoxide Dismutase [Bovine erythrocytes] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
Western Blot of HRP Conjugated Superoxide Dismutase Antibody. Lane 1: Superoxide Dismutase Load: 50 ng per lane. Primary antibody: HRP Conjugated Superoxide Dismutase Antibody at 1:1000 overnight at 4°C. Secondary antibody: none Detected directly using FEMTOMAX Substrate. Block: orb348637 for 30 min at RT. Predicted/Observed size: 15.6 kDa, 15.6 kDa.
Equine | |
0.78-50 ng/mL | |
0.21 ng/mL |