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Superoxide Dismutase Antibody Peroxidase Conjugated

Catalog Number: orb344919

DispatchUsually dispatched within 5-10 working days
$ 690.00
Catalog Numberorb344919
CategoryAntibodies
DescriptionSuperoxide Dismutase antibody (Peroxidase)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityBovine
IsotypeIgG
ImmunogenSuperoxide Dismutase [Bovine erythrocytes]
Antibody TypePrimary Antibody
Concentration1.0 mg/mL
Dilution rangeELISA: 1:5,000 - 1:20,000, WB: 1:500- 1:2,000
Form/AppearanceLyophilized
PuritySuperoxide Dismutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Superoxide Dismutase [Bovine erythrocytes]. Cross reactivity against Superoxide Dismutase from other tissues and species may occur but have not been specifically determined.
ConjugationHRP
UniProt IDP00442
NCBIDAA33706.1
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Alternative namesrabbit anti-Superoxide Dismutase 1 Antibody HRP co
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NoteFor research use only
Application notesAnti-Superoxide Dismutase Peroxidase Conjugated Antibody has been tested by Western blot and is suitable to be assayed against 1.0 ug of Superoxide Dismutase [Bovine erythrocytes] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.
Expiration Date12 months from date of receipt.
Superoxide Dismutase Antibody Peroxidase Conjugated

Western Blot of HRP Conjugated Superoxide Dismutase Antibody. Lane 1: Superoxide Dismutase Load: 50 ng per lane. Primary antibody: HRP Conjugated Superoxide Dismutase Antibody at 1:1000 overnight at 4°C. Secondary antibody: none Detected directly using FEMTOMAX Substrate. Block: orb348637 for 30 min at RT. Predicted/Observed size: 15.6 kDa, 15.6 kDa.