Superoxide Anion Colorimetric Assay Kit

SKU: orb1173205

Description

The kit provides a method for detecting the O2- content in biological samples such as tissues, serum, and cells. The superoxide anion reacts with hydroxylamine hydrochloride to produce NO2-, which then reacts with Gris' reagent.

Images & Validation

• Application Notes:
  The kit provides a simple method for detecting the O2- content in a variety of biological samples such as animal tissues, plant tissues, serum, cells . In the assay, The superoxide anion in variety of biological samples reacts with hydroxylamine hydrochloride to produce NO2－, and NO2－ reacts with Gris’ reagent. The mechanism of Gris’ analysis is summarized as the azo coupling between diazoniums, which is It is produced by sulfonamides and NO2- and N-(1-naphthyl)ethylenediamine dihydrochloride to generate a red azo compound with a characteristic absorption peak at 540nm. The O2- content in the sample can be calculated based on the A540 value.

Key Properties

• Range: 0.0125-0.5 mg/mL
• Sensitivity: 0.0125 mg/mL

Storage & Handling

• Storage: Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
• Expiration Date: 6 months from date of receipt.
• Hazard Information: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
• Disclaimer: For research use only