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Catalog Number | orb1252093 |
---|---|
Category | Antibodies |
Description | SUMO2 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | S2MT-1 |
Tested applications | IF, IHC-P |
Reactivity | Human, Rat |
Isotype | IgG1, kappa |
Immunogen | Recombinant human protein was used as the immunogen for the SUMO-2 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Immunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the SUMO-2 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | SUMO2 |
UniProt ID | P61956 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | SUMO2/3 Antibody: HSMT3, SMT3B, SUMO3, Smt3A, SMT3 Read more... |
Note | For research use only |
Application notes | Immunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the SUMO-2 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human tonsil stained with SUMO-2 antibody
Formalin-fixed, paraffin-embedded rat ovary stained with SUMO-2 antibody.
IF, IHC-P, WB | |
Hamster, Other, Porcine, Zebrafish | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Hamster, Mouse, Other, Porcine, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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