You have no items in your shopping cart.
Page Not Found
Cart summary
Item 1 of 3
Item 1 of 3
Sulfotyrosine Antibody: Biotin
Catalog Number: orb396795
| Catalog Number | orb396795 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against Sulfotyrosine conjugated to Biotin. |
| Target | Sulfotyrosine |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | IgG1 |
| Conjugation | Biotin |
| Reactivity | All |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS |
| Purification | Protein G Purified |
| Immunogen | Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Tested applications | ELISA, ICC, IF, IHC, WB |
| Dilution range | WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC (1:25) |
| Application notes | A 1:1000 dilution of SMC-522 was sufficient for detection of Sulfotyrosine in 0.5 µg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. |
| Clone Number | 12B11 (Formerly sold as 7C5) |
| Storage | Conjugated antibodies should be stored according to the product label |
| Alternative names | Sulfotyrosine antibody, Tyrosine sulfation antibod Read more... |
| Note | For research use only |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 12B11. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody. (D) Composite.
Immunohistochemistry analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 12B11. Tissue: kidney. Species: Human. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody (D) Composite.
Western Blot analysis of Sulfotyrosine-BSA Conjugate showing detection of 67 kDa Sulfotyrosine protein using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 12B11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Sulfotyrosine-BSA. Lane 3: Tyrosine-BSA. Lane 4: Phosphotyrosine-BSA. Lane 5: BSA. Load: 2 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
