Streptavidin Phycoerythrin Conjugated

SKU: orb348769

Description

Streptavidin Phycoerythrin Conjugated

Research Area

Non-Animal

Key Properties

• Purity: Streptavidin-Phycoerythrin was prepared from electrophoretically purified streptavidin isolated from Streptomyces avidinii conjugated to the chromophore R-Phycoerythrin. Free fluorochrome is removed by tandem molecular sieve chromatography.
• Conjugation: RPE

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.5 mg/mL
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

Streptavidin PE, Phyco Streptavidin, SA Phycoerythrin, R-Phycoerythrin, Phycoerythrin conjugated streptavidin

AL-57 IgG4 and ICAM-1 bind to the activated HA form of LFA-1 on PBMCs. Binding of AL-57 IgG4 (A) and ICAM-1 (B) to PBMCs with inactivating buffer (CaCl2/MgCl2) or with activating buffer (MgCl2/EGTA) in the presence or absence of PMA (10 ng/mL) or DTT (500 µm) was determined by flow cytometric analysis as described in Materials and Methods. The concentration of each IgG or ICAM-1 used was 10 µg/ml. For AL-57 staining, Neg. Control indicates negative control with just the secondary PE-labeled streptavidin (p/n orb348769) antibody used for staining; MHM24 was used as a control that did not distinguish between the HA and LA forms of LFA-1. For ICAM-1 staining, a multimeric complex [ICAM-1/streptavidin (SV)-PE] of biotinylated human ICAM-1-Fc and PE-labeled streptavidin was used; SV-PE only indicates negative control with just the PE-labeled streptavidin used for staining. Shown here are representative histograms of samples under indicated conditions. The x-axis depicts the fluorescence intensity of individual cells, and the y-axis represents the cell number. The numbers shown are relative values of MFI with relative percentages of positive cells in parenthesis.

Anti-FLAG DyLight™ 649 conjugate - X-axis and Streptavidin-PE conjugate - Y-axis (p/n orb348769) are used in FACS sorting of yeast surface display camelid VHH immunized library to isolate yeast clones expressing antibodies to a target antigen. VHH antibodies displayed on yeast cell surface are tagged with FLAG epitope. Yeast library was doubly stained with Anti-FLAG DyLight™ 649 conjugate to monitor VHH expression and biotinylated antigen/Streptavidin-PE conjugate complexes to detect VHH binding to the antigen. Double positive yeast cells were isolated using FACSaria II cell sorter.

Cytoflow of Streptavidin-Phycoerythrin (p/n orb348769). Cells: Yeast Saccharomyces cerevisiae cells expressing surface receptor protein. [LEFT] unstained; [MIDDLE] Streptavidin-Phycoerythrin at 1:200 (negative control); [RIGHT] Streptavidin-Phycoerythrin at 1:200 with biotinylated ligand test sample.

Dot Blot of Streptavidin Phycoerythrin conjugate. Row A: BSA [10 mg/ml]. Row B: BSA/Biotin [1.0 mg/ml]. Column Dilutions: 100 ng, 33.33 ng, 11.11 ng, 3.70 ng, 1.23 ng. Primary Antibody: Streptavidin Phycoerythrin Conjugate at 1 µg/ml for 1hr at RT. Secondary Antibody: None. Blocking: BlockOut Buffer (p/n orb348644).

Quick Database Links

UniProt

P22629

RefSeq:CAA00084.1