Streptavidin antibody

• Catalog Number: orb344582
• Category: Antibodies
• Description: Streptavidin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Streptavidin [Streptomyces avidinii]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:200,000, IHC: 1:1,500 - 1:5,000, WB: 1:3,000 - 1:12,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Streptavidin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii]. No cross reactivity occurs against Avidin.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Streptavidin Antibody, rabbit anti Str
• Note: For research use only
• Application notes: Anti-Streptavidin antibody has been tested by ELISA, SDS-Page, and dot blot and is suitable for western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes using