Streptavidin antibody (FITC)

• Catalog Number: orb344714
• Category: Antibodies
• Description: Streptavidin antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Streptavidin (Streptomyces avidinii)
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: User Optimized, FC: User Optimized, IF: 1:500-1:2,500
• Form/Appearance: Lyophilized
• Purity: Anti-Streptavidin Antibody Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.
• Conjugation: FITC
• Biological Activity: 4.72
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Streptavidin Antibody FITC Conjugation
• Note: For research use only
• Application notes: Anti-Streptavidin Antibody Fluorescein Conjugated has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions using Streptavidin antibody (FITC)