Streptavidin antibody (Biotin)

• Catalog Number: orb344755
• Category: Antibodies
• Description: Streptavidin antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Streptavidin (Streptomyces avidinii)
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000-1:20,000
• Form/Appearance: Lyophilized
• Purity: Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.
• Conjugation: Biotin
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Streptavidin Antibody biotin Conjugati
• Note: For research use only
• Application notes: Biotin Conjugated Anti-Streptavidin Antibody has been tested by dot blot and is suitable to be assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Optimization of the concentration in immunoassays should be performed by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Trypsin Inhibitor reduced (Lane1), Trypsin Inhibitor non-reduced (Lane2), Streptavidin reduced (Lane3), Streptavidin non-reduced (Lane4) Load: ~1ug per lane using Streptavidin antibody (Biotin)